Sequencing data quality
Results
*Based on quality control of the sequencer with DNBSEQ ™ technology
In this section you will find a compilation of much of the data generated by our sequencer with DNA nanoball technology, including graphs and tables on base distribution, read quality and array efficiency.
COMPARATIVE STUDIES OF DNBSEQ™ TECHNOLOGY
Rao, J. et al. assessed the efficacy for detecting copy number variations (CNV) using whole-genome sequencing with the DNBSEQ™ platform. In previous studies they had verified the platform’s efficacy for detecting single-nucleotide variants (SNVs) and insertions/deletions; this study focused on CNV detection. Results indicate that CNV detection capability on DNBSEQ™ is comparable to other platforms in terms of quantity, length, distribution, sensitivity and genome-wide accuracy. Moreover, DNBSEQ™ platforms are highlighted as providing a more accurate view of smaller CNVs.
Rao, J., Peng, L., Liang, X. et al. (2020). Performance of copy number variants detection based on whole-genome sequencing by DNBSEQ platforms. BMC Bioinformatics 21, 518.
ABRF conducted a comparative study to assess the reproducibility, accuracy and usefulness of several NGS sequencing platforms. Among them, instruments with DNBSEQ ™ technology showed the lowest sequencing error and read-duplication rates of all instruments assessed. Samples used were both human and bacterial DNA, on which DNBSEQ™ instruments showed high data quality and broad coverage. Variant analysis also revealed greater detection of SNPs and INDELs with very low error rates, being especially accurate in exonic regions. They also showed higher accuracy and sensitivity, with the lowest number of false positives and the highest detection of Mendelian inconsistencies.
Click here to see the results.
YFoox, J.et al. Performance assessment of DNA sequencing platforms in the ABRF Next-Generation Sequencing Study. Nat Biotechnol 39, 1129–1140 (2021).
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