The Association of Biomolecular Resource Facilities (ABRF) conducted a comparative study to assess the reproducibility, accuracy and usefulness of several NGS sequencing platforms. Among them, short-read sequencing instruments with DNBSEQ ™ technology showed the lowest sequencing error rates of all instruments. Samples used were both human and bacterial DNA, and DNBSEQ™ platforms stood out for data quality and broad coverage, with data highly consistent across all libraries. Such data included high base-quality scores, GC distributions, balanced sequence content and low levels of both N content and sequence duplication. YFoox, J., Tighe, S.W., Nicolet, C.M. et al. Performance assessment of DNA sequencing platforms in the ABRF Next-Generation Sequencing Study. Nat Biotechnol 39, 1129–1140 (2021).